新孢子虫病是由犬新孢子虫引起的农场动物和狗的一种经证实的疾病。这项横断面研究调查了268只狗中的N.caninum患病率和血清阳性率。对狗粪便进行Nc5基因PCR并通过测序确认。使用间接荧光抗体测试(IFAT)检测血清阳性率。三个年龄组,性别,局部性(安曼,Irbid,和扎尔卡省),狗类型(流浪,宠物,和繁殖),居住的地方(室内/室外),食物类型(生/熟),腹泻,在该地区堕胎,使用单变量和多变量逻辑回归分析,将附近的动物作为独立变量与犬奈瑟氏菌阳性相关。使用Nc5-PCR测试,犬根虫的真实患病率为34.3%(95%CI28.4,40.5)。使用IFAT,约旦犬之间犬的真实血清阳性率为47.9%(95%CI41.4,54.5)。Nc5-PCR产物的测序分离株(n=85)与三个犬奈瑟菌菌株相匹配,即,NcHareGre(n=70,82.4%,95%CI72.6-89),NCMS2(n=14,16.5%,95%CI9.3-26.1),和L218(n=1,1.2%,95%CI0.03-6.4)。这三个菌株以前是从三个不同的国家和大陆分离的。N.caninum脱落与该地区狗和动物的流产有关(比值比=3.6)。在安曼和扎尔卡,住在室内降低血清阳性率为0.45、0.24和0.02,分别。约旦与三个不同的国家和大洲共享三种分子犬毒株。
Neosporosis is a proven disease of farm animals and dogs caused by Neospora caninum. This cross-sectional study investigates N. caninum prevalence and seroprevalence among 268 dogs. Nc5 gene PCR was carried out on dog faeces and confirmed by sequencing. Seroprevalence was detected using an indirect fluorescent antibody test (IFAT). Three age groups, gender, locality (Amman, Irbid, and Zarqa Governorates), dog type (stray, pet, and breeding), place of living (indoor/outdoor), food type (raw/cooked), having diarrhoea, having abortion in the area, and having animals nearby were tested as independent variables for associations with positivity to N. caninum using univariate and multivariable logistic regression analyses. The true prevalence of N. caninum was 34.3% (95% CI 28.4, 40.5) using the Nc5-PCR test. The true seroprevalence rate of N. caninum among dogs in Jordan was 47.9% (95% CI 41.4, 54.5) using IFAT. The sequenced isolates of Nc5-PCR products (n = 85) matched three N. caninum strains, namely, NcHareGre (n = 70, 82.4%, 95% CI 72.6-89), NC MS2 (n = 14, 16.5%, 95% CI 9.3-26.1), and L218 (n = 1, 1.2%, 95% CI 0.03-6.4). The three strains were isolated previously from three different countries and continents. N. caninum shedding is associated with abortion among dogs and animals in the area (odds ratio = 3.6). In Amman and Zarqa, living indoors reduced seroprevalence at 0.45, 0.24, and 0.02 odds ratios, respectively. Jordan shares three molecular N. caninum strains with three different countries and continents.