桃棕腐烂,归因于果莫尼氏菌,对采后桃种植构成重大威胁,造成高达80%的损失。随着越来越多的国家,由欧盟带头,对水果生产中的化学制剂实施禁令,从生物防治菌株中挖掘高活性抗菌化合物用于采后疾病管理的兴趣与日俱增。在这项研究中,我们强调了林肯链霉菌菌株JCP1-7抑制果果分枝杆菌孢子形成的独特能力,尽管其抗菌功效有限。通过GC-MS分析,桉树脑被确定为关键化合物。用浓度为0.0335μgcm-3的桉树脑熏蒸患病果实,对果果霉菌的体内抑制率为93.13%,完全抑制孢子形成。转录组分析显示桉树脑对多种发病相关途径的影响,特别是通过抑制过氧化氢酶2(Cat2)的表达。用MfCat2敲除菌株(ΔMfCat2)进行的实验显示,对JCP1-7和桉树脑的致病性和敏感性降低,表明MfCat2是JCP1-7和桉树脑对抗果果分枝杆菌的潜在靶标。我们的发现阐明了由S.lincolnensisJCP1-7产生的桉树脑通过调节MfCat2抑制果粒孢子形成,从而有效地减少了采后桃棕腐烂的发生。桉树脑熏蒸的使用提供了对桃棕腐烂大规模管理的见解,从而为农业研究做出了重大贡献。
Peach brown rot, attributed to Monilinia fructicola, presents a significant threat to postharvest peach cultivation, causing losses of up to 80%. With an increasing number of countries, spearheaded by the European Union, imposing bans on chemical agents in fruit production, there is a growing interest in mining highly active antibacterial compounds from biological control strains for postharvest disease management. In this study, we highlight the unique ability of Streptomyces lincolnensis strain JCP1-7 to inhibit M. fructicola sporulation, despite its limited antimicrobial efficacy. Through GC-MS analysis,
eucalyptol was identified as the key compound. Fumigation of diseased fruits with
eucalyptol at a concentration of 0.0335 μg cm-3 demonstrated an in vivo inhibition rate against M. fructicola of 93.13%, completely suppressing spore formation. Transcriptome analysis revealed the impact of eucalyptol on multiple pathogenesis-related pathways, particularly through the inhibition of catalase 2 (Cat2) expression. Experiments with a MfCat2 knockout strain (ΔMfCat2) showed reduced pathogenicity and sensitivity to JCP1-7 and eucalyptol, suggesting MfCat2 as a potential target of JCP1-7 and
eucalyptol against M. fructicola. Our findings elucidate that
eucalyptol produced by S. lincolnensis JCP1-7 inhibits M. fructicola sporulation by regulating MfCat2, thereby effectively reducing postharvest peach brown rot occurrence. The use of fumigation of
eucalyptol offers insights into peach brown rot management on a large scale, thus making a significant contribution to agricultural research.