背景:尽管阴茎的神经和血管在勃起中起重要作用,关于它们在人类胎儿中发育的研究很少。因此,本研究的目的是分析,定量,在海绵体和海绵体中,不同胎龄胎儿阴茎神经和血管的发育。
方法:56个新鲜,使用年龄在受孕后13至36周(WPC)的宏观正常人类胎儿。妊娠年龄由脚长标准确定。阴茎立即固定在10%福尔马林中,常规处理石蜡包埋,之后从中轴获得组织切片。我们使用免疫组织化学染色来分析海绵体和海绵体中的神经和血管。通过使用Image-J软件将这些元素鉴定并定量为百分比。
结果:定量分析显示,海绵体的神经百分比从3.03%到20.35%,海绵体的神经百分比从1.89%到23.88%。线性回归分析表明,海绵体和海绵体的神经生长(发生率)与胎儿年龄显着正相关(r2=0.9421,p<0.0001)和(r2=0.9312,p<0.0001)。分别,在整个胎儿期研究。此外,定量分析显示,海绵体中血管的百分比从2.96%到12.86%不等,海绵体中血管的百分比从3.62%到14.85%不等。线性回归分析表明,海绵体和海绵体的血管生长(外观)与胎儿年龄显着正相关(r2=0.8722,p<0.0001)和(r2=0.8218,p<0.0001)。分别,在整个胎儿期研究。此外,线性回归分析表明,在妊娠中期,海绵体神经的生长速度更快,与海绵体的神经相比。此外,线性回归分析表明,与海绵体相比,海绵体中血管的增长率更高,在整个胎儿期研究。
结论:在胎儿期,人类的阴茎经历了重大的发育变化,尤其是神经和血管的含量和分布。我们发现神经和血管生长(数量)与胎儿年龄之间有很强的相关性,在海绵体和海绵体中。在研究的整个胎儿期,神经的比例比血管的比例要大得多。此外,神经和血管的生长速度比海绵体和海绵体区域的生长速度更快。
BACKGROUND: Although nerves and vessels of the penis play important role in erection, there are few studies on their development in human fetus. Therefore, the objective of the present study is to analyze, quantitatively, in the corpora cavernosa and corpus spongiosum, the development of the nerves and vessels in the fetal penis at different gestational ages.
METHODS: Fifty-six fresh, macroscopically normal human fetuses aged from 13 to 36 weeks post-conception (WPC) were used. Gestational age was determined by the foot length criterion. Penises were immediately fixed in 10% formalin, and routinely processed for paraffin embedding, after which tissue sections from the mid-shaft were obtained. We used immunohistochemical staining to analyze the nerves and vessels in the corpus cavernous and in the corpus spongiosum. These elements were identified and quantified as percentage by using the Image-J software.
RESULTS: The quantitative analysis showed that the percentage of nerves varied from 3.03% to 20.35% in the corpora cavernosa and from 1.89% to 23.88% in the corpus spongiosum. The linear regression analysis indicated that nerves growth (incidence) in the corpora cavernosa and corpus spongiosum correlated significantly and positively with fetal age (r2=0.9421, p<0.0001) and (r2=0.9312, p<0.0001), respectively, during the whole fetal period studied. Also, the quantitative analysis showed that the percentage of vessels varies from 2.96% to 12.86% in the corpora cavernosa and from 3.62% to 14.85% in the corpus spongiosum. The linear regression analysis indicated that vessels growth (appearance) in the corpora cavernosa and corpus spongiosum correlated significantly and positively with fetal age (r2=0.8722, p<0.0001) and (r2=0.8218, p<0.0001), respectively, during the whole fetal period studied. In addition, the linear regression analysis demonstrated a more intense growth rate of nerves in the corpus spongiosum during the 2nd trimester of gestation, when compared with nerves in the corpora cavernosa. In addition, the linear regression analysis demonstrated a more intense growth rate of vessels in the corpus spongiosum when compared with the corpora cavernosa, during the whole fetal period studied.
CONCLUSIONS: In the fetal period, the human penis undergoes major developmental changes, notably in the content and distribution of nerves and vessels. We found strong correlation between nerves and vessels growth (amount) with fetal age, both in the corpora cavernosa and corpus spongiosum. There is significant greater proportional number of nerves than vessels during the whole fetal period studied. Also, nerves and vessels grow in a more intense rate than that of the corpora cavernosa and corpus spongiosum areas.