传统的皮肤取样方法包括打孔或剃须活检以产生用于分析的实体组织样品。这些活检程序很痛苦,需要麻醉,留下永久的伤疤.本单元描述了一种抽吸水泡皮肤活检方法,可用于代替传统的活检方法,无疤痕皮肤取样技术。抽吸水泡的诱导使用具有向皮肤施加负压和温和热量的腔室的仪器。泡罩形成发生在1小时内,产生多达五个水泡,每个活检部位直径10毫米。可以提取泡状液并离心以从表皮和上真皮中检索细胞进行流式细胞术,单细胞RNA测序,细胞培养,更不需要消化方案。此外,泡状流体可用于测量可溶性蛋白质和代谢物。本单元描述了用品和主题的准备,抽吸泡罩活检程序和泡罩形成,流体萃取,和起泡后的护理。©2024Wiley期刊有限责任公司。基本方案1:用品和受试者的准备基本方案2:抽吸泡罩活检程序和形成基本方案3:泡罩流体提取基本方案4:泡罩后护理和清洁。
Traditional skin sampling methods include punch or shave biopsies to produce a solid tissue sample for analysis. These biopsy procedures are painful, require anesthesia, and leave permanent scars. This unit describes a suction
blister skin biopsy method that can be used in place of traditional biopsy methodologies as a minimally invasive, non-scarring skin sampling technique. The induction of suction blisters uses an instrument with a chamber that applies negative pressure and gentle heat to the skin. Blister formation occurs within 1 hr, producing up to five blisters, each 10 mm in diameter per biopsy site.
Blister fluid can be extracted and centrifuged to retrieve cells from the epidermis and upper dermis for flow cytometry, single-cell RNA sequencing, cell culture, and more without the need for digestion protocols. In addition, the
blister fluid can be used to measure soluble proteins and metabolites. This unit describes the preparation of supplies and subjects, the suction blister biopsy procedure and
blister formation, fluid extraction, and post-blistering care. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Preparation of supplies and subject Basic Protocol 2: Suction blister biopsy procedure and formation Basic Protocol 3:
Blister fluid extraction Basic Protocol 4: Post-
blister care and clean up.