背景:本研究旨在鉴定失调的基因,分子途径,和人乳头瘤病毒(HPV)相关宫颈癌的调节机制。我们研究了疾病相关基因以及基因本体论,生存预后,转录因子和微小RNA(miRNA)参与宫颈癌的发生,能够更深入地理解与HPV相关的宫颈癌。
方法:我们使用10个可公开获取的基因表达综合(GEO)数据集来检查宫颈癌中的基因表达模式。差异表达基因(DEGs),这显示了宫颈癌和健康组织样本之间的明显区别,使用GEO2R工具进行分析。使用其他生物信息学技术进行途径分析和功能富集,以及分析基因表达改变与HPV感染之间的联系。
结果:总计,与健康组织相比,48个DEGs被鉴定为在宫颈癌组织中差异表达。在DEG中,CCND1、CCNA2和SPP1是HPV相关宫颈癌的关键失调基因。针对这些基因鉴定的五种常见miRNA是miR-7-5p,miR-16-5p,miR-124-3p,miR-10b-5p和miR-27a-3p。miRNAhsa-miR-27a-3p靶向的hub-DEG受共同转录因子SP1控制。
结论:本研究已经确定了参与HPV相关宫颈癌进展的DEGs以及调节它们的各种分子途径和转录因子。这些发现使人们更好地了解宫颈癌,从而开发和确定可能的治疗和干预目标。分别。
BACKGROUND: The present study aimed to identify dysregulated genes, molecular pathways, and regulatory mechanisms in human papillomavirus (HPV)-associated cervical cancers. We have investigated the disease-associated genes along with the Gene Ontology, survival prognosis, transcription factors and the microRNA (miRNA) that are involved in cervical carcinogenesis, enabling a deeper comprehension of cervical cancer linked to HPV.
METHODS: We used 10 publicly accessible Gene Expression Omnibus (GEO) datasets to examine the patterns of gene expression in cervical cancer. Differentially expressed genes (DEGs), which showed a clear distinction between cervical cancer and healthy tissue samples, were analyzed using the GEO2R tool. Additional bioinformatic techniques were used to carry out pathway analysis and functional enrichment, as well as to analyze the connection between altered gene expression and HPV infection.
RESULTS: In total, 48 DEGs were identified to be differentially expressed in cervical cancer tissues in comparison to healthy tissues. Among DEGs, CCND1, CCNA2 and SPP1 were the key dysregulated genes involved in HPV-associated cervical cancer. The five common miRNAs that were identified against these genes are miR-7-5p, miR-16-5p, miR-124-3p, miR-10b-5p and miR-27a-3p. The hub-DEGs targeted by miRNA hsa-miR-27a-3p are controlled by the common transcription factor SP1.
CONCLUSIONS: The present study has identified DEGs involved in HPV-associated cervical cancer progression and the various molecular pathways and transcription factors regulating them. These findings have led to a better understanding of cervical cancer resulting in the development and identification of possible therapeutic and intervention targets, respectively.