{Reference Type}: Comparative Study
{Title}: Comparison of repair of methylated pyrimidines in poly(dT) by extracts from rat liver and Escherichia coli.
{Author}: Dolan ME;Scicchitano D;Singer B;Pegg AE;
{Journal}: Biochem Biophys Res Commun
{Volume}: 123
{Issue}: 1
{Year}: Aug 1984 30
{Factor}: 3.322
{DOI}: 10.1016/0006-291x(84)90416-9
{Abstract}: Partially purified preparations of O6-alkylguanine-DNA alkyltransferase from rat liver and E. coli were tested for their ability to repair O4-methylthymine in a methylated poly(dT) X poly(dA) substrate. The bacterial preparation readily carried out this reaction, but no loss of O4-methylthymine was obtained with the rat liver protein. These results indicate a significant difference in specificity between the mammalian and bacterial proteins which could have important consequences for carcinogenesis and mutagenesis by alkylating agents in mammalian cells.