{Reference Type}: Journal Article
{Title}: Bioactive substance contents and therapeutic potential for skin inflammation of an herbal gel containing Derris reticulata and Glycyrrhiza glabra.
{Author}: Sangkaew W;Sianglum W;Wunnoo S;Voravuthikunchai SP;Joycharat N;
{Journal}: Pharm Biol
{Volume}: 62
{Issue}: 1
{Year}: 2024 Dec
{Factor}: 3.889
{DOI}: 10.1080/13880209.2024.2385456
{Abstract}: <B>UNASSIGNED: </B>Derris reticulata Craib. and Glycyrrhiza glabra L., of the Fabaceae, have been used as active components in Thai herbal formulas for the treatment of fever and skin diseases.<BR><B>UNASSIGNED: </B>To evaluate the physicochemical and pharmacological properties of the developed herbal gel formulation containing the combined extract from D. reticulata stem wood and G. glabra root (RGF).<BR><B>UNASSIGNED: </B>The potential of the herbal gel formulation containing RGF (8% w/w) as the active ingredient was studied by evaluating the anti-inflammatory, antioxidant, and anti-Staphylococcus aureus activities using quantitative reverse transcription-polymerase chain reaction assay, spectrophotometric method, and broth microdilution technique, respectively. The reference standards for the biological testing included Nω-nitro-L-arginine (L-NA), ascorbic acid, catechin, and penicillin G. The stability study of the RGF herbal gel was performed by a heating-cooling test (at 45 °C for 24 h and at 4 °C for 24 h/1 cycle; for 6 cycles), and the bioactive marker compounds in the herbal gel were investigated by the HPLC technique.<BR><B>UNASSIGNED: </B>RGF showed promising pharmacological effects, particularly on its anti-inflammatory property (IC50 73.86 µg/mL), compared to L-NA (IC50 47.10 µg/mL). The RGF-containing gel demonstrated anti-inflammatory (IC50 3.59 mg/mL) and free radical scavenging effects (IC50 0.05-4.39 mg/mL), whereas it had no anti-S. aureus activity (MIC > 10 mg/mL). The active ingredient in the developed herbal gel significantly inhibited lipopolysaccharide-induced nitric oxide production by downregulating iNOS mRNA levels. The contents of the bioactive markers in the RGF gel (lupinifolin and glabridin) did not change significantly after stability testing.<BR><B>UNASSIGNED: </B>The RGF-containing gel has potential to be further developed as an herbal product for the treatment of skin inflammation.