{Reference Type}: Journal Article
{Title}: Plasticity-induced actin polymerization in the dendritic shaft regulates intracellular AMPA receptor trafficking.
{Author}: Wong VC;Houlihan PR;Liu H;Walpita D;DeSantis MC;Liu Z;O'Shea EK;
{Journal}: Elife
{Volume}: 13
{Issue}: 0
{Year}: 2024 Aug 15
{Factor}: 8.713
{DOI}: 10.7554/eLife.80622
{Abstract}: AMPA-type receptors (AMPARs) are rapidly inserted into synapses undergoing plasticity to increase synaptic transmission, but it is not fully understood if and how AMPAR-containing vesicles are selectively trafficked to these synapses. Here, we developed a strategy to label AMPAR GluA1 subunits expressed from their endogenous loci in cultured rat hippocampal neurons and characterized the motion of GluA1-containing vesicles using single-particle tracking and mathematical modeling. We find that GluA1-containing vesicles are confined and concentrated near sites of stimulation-induced structural plasticity. We show that confinement is mediated by actin polymerization, which hinders the active transport of GluA1-containing vesicles along the length of the dendritic shaft by modulating the rheological properties of the cytoplasm. Actin polymerization also facilitates myosin-mediated transport of GluA1-containing vesicles to exocytic sites. We conclude that neurons utilize F-actin to increase vesicular GluA1 reservoirs and promote exocytosis proximal to the sites of synaptic activity.