{Reference Type}: Journal Article {Title}: Application of the Biological Toxicity Effect Ratio (BER) Method for Advancing Water Quality Criteria Derivation. {Author}: Ding R;Wu Y;Liao Z;Lu Y;Wei D;Chen Z;Hu H; {Journal}: Environ Sci Technol {Volume}: 0 {Issue}: 0 {Year}: 2024 Aug 15 {Factor}: 11.357 {DOI}: 10.1021/acs.est.4c06110 {Abstract}: Water quality criteria (WQC) serve as a scientific foundation for pollutant risk assessment and control in aquatic ecosystems. The development of regionally differentiated WQC tailored to specific regional characteristics has become an emerging trend. However, the current WQC is constrained by a lack of regional species toxicity data. To address these limitations, this study proposes the biological toxicity effect ratio (BER) method, which indirectly reflects the toxicity sensitivity of the overall aquatic ecosystem through the toxicity information on a limited number of species, enabling rapid WQC prediction. Using the established WQC in China and the USA as a case study, we combined mathematical derivation and data validation to evaluate the BER method. Among various species-taxon groups of freshwater organisms, planktonic crustaceans demonstrated the highest predictive accuracy. Our analysis further revealed that species toxicity sensitivity and regional variability jointly influence the prediction accuracy. Regardless of the evaluation indexes, planktonic crustaceans emerged as the most suitable species-taxon group for the BER method. Additionally, the BER method is particularly applicable to pollutants with conserved mechanisms across species. This study systematically explores the feasibility of using the BER method and offers new insights for deriving regionally differentiated WQC.