{Reference Type}: Journal Article
{Title}: Association of adiponectin gene single nucleotide polymorphisms with environmental risk factors in type 2 diabetes mellitus: An updated evidence of haplotype-based analysis study.
{Author}: Mamashli E;Goulding RP;Iranparvar M;Skishahr FS;Siahkouhian M;Ramezanzade R;Jaspers RT;Davarnia B;
{Journal}: Gene
{Volume}: 0
{Issue}: 0
{Year}: 2024 Aug 9
{Factor}: 3.913
{DOI}: 10.1016/j.gene.2024.148816
{Abstract}: <B>OBJECTIVE: </B>Adiponectin (ADIPOQ) gene is considered to be one of the promising players in deciphering the genetic bases of type 2 diabetes. This study investigated the associations between haplotype combinations of three single nucleotide polymorphisms (SNPs) of the ADIPOQ gene and two SNPs of the ADIPOQ receptor genes with environmental risk factors for the prediction of T2DM disorder susceptibility in the Iranian population.<BR><B>METHODS: </B>This case-control and cross-sectional study was conducted on 182 patients with T2DM and 155 healthy controls. Genotyping was performed using amplification refractory mutation system-PCR (ARMS-PCR) for rs17300539G/A, rs2241766T/G, and rs1501299G/T of the ADIPOQ gene, rs1342387C/T of the AdipoR1 gene, and rs10773989T/C of the AdipoR2 gene.<BR><B>RESULTS: </B>All polymorphisms met the Hardy-Weinberg equilibrium (p > 0.05). The studied SNPs; rs17300539, rs2241766 of ADIPOQ gene and rs10773989of AdipoR2 gene, were significantly associated with increased risk of T2DM. Two-way ANOVA analysis indicated that GG carriers of rs2241766T/G had a significantly lower waist-to-hip ratio and body mass index compared to TT carriers, and also GG of rs2241766T/G showed the greatest HbA1c levels compared to any other genotype. CC carriers of rs10773989T/C displayed a significantly higher LDL level compared to the other two genotype carries. According to Combined Haplotype ([rs17300539, rs2241766, rs1501299] / [rs17300539, rs2241766, rs1501299]) analysis, GTT- homozygote carriers displayed the highest plasma adiponectin levels. In contrast, GGG/GTG, ATG/GTG, and GGG/GGG showed the lowest plasma adiponectin levels in the controls.<BR><B>CONCLUSIONS: </B>The adiponectin gene haplotype combinations were associated with plasma adiponectin concentrations in healthy people. In T2DM, adiponectin genetic variants displayed less effect on adiponectin plasma concentrations.