{Reference Type}: Journal Article
{Title}: Rapamycin-Induced Translocation of Meiotic Nuclear Proteins in Saccharomyces cerevisiae.
{Author}: Padmarajan J;Edilyam AK;Subramanian VV;
{Journal}: Methods Mol Biol
{Volume}: 2818
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3906-1_14
{Abstract}: Conditional depletion of proteins is a potential strategy to elucidate protein function, especially in complex cellular processes like meiosis. Several methods are available to effectively deplete a protein in a conditional manner. Conditional loss of a protein function can be achieved by depleting it from its region of action by degrading it. A conditional loss of protein function can also be achieved by sequestering it to a functionally unavailable compartment inside the cell. This chapter describes anchor away, a conditional depletion tool that can deplete a protein both temporally and spatially by translocation. It utilizes the affinity of FRB to bind FKBP12 in the presence of rapamycin for a quick and efficient translocation of the protein to a designated location. Anchor away is a reliable tool for the study of meiotic proteins, as only small quantities of rapamycin are required to efficiently and rapidly translocate the protein of interest without compromising meiotic progression.