{Reference Type}: Journal Article
{Title}: Immunofluorescence and biochemical investigation of the protective effects of naringin and diosmin on the freezability of merino ram semen.
{Author}: Omur AD;Kaynar O;Iieriturk M;Ozkaraca M;Ozturk AE;Bodu M;Sahin IN;Akarsu SA;
{Journal}: Cryo Letters
{Volume}: 45
{Issue}: 5
{Year}: 2024 Sep-Oct
{Factor}: 0.892
{Abstract}: <B>BACKGROUND: </B>Various antioxidant substances are added to sperm extenders to protect spermatozoa against oxidative stress and cryodamage.<BR><B>OBJECTIVE: </B>To investigate the effects of the flavonoid diosmin (DIO) and a flavanone glycoside naringin (NAR) on the freezability of ram semen.<BR><B>METHODS: </B>In this study, six Merino rams were used during the breeding season. The ejaculates were pooled after collection from the rams. Pooled ejaculates were divided into six groups: control, NAR 1 mM, NAR 2 mM, NAR 4 mM, DIO 2 mM, and DIO 4 mM, and then diluted with a TRIS-based diluent. The pooled semen was equilibrated, placed in 0.25 mL pipettes with 10 × 10 7 sperm cells in each pipette, and frozen in liquid nitrogen vapor. After 24 h, the pipettes were thawed at 37 degree C for 25 s and analyzed in terms of spermatological parameters.<BR><B>RESULTS: </B>The highest plasma membrane integrity ratio was found in the DIO 4 mM group, whereas a statistically significant difference was found between the NAR 1 mM and NAR 2 mM groups (p < 0.05). While the DIO 4 mM group had the highest acrosome integrity rate, a statistically significant difference was found between the other groups (p < 0.05). Mitochondrial activity was the highest in the NAR 4 mM, DIO 4 mM and DIO 2 mM groups (p < 0.05). In the analysis of the sperm membrane lipid profile, it was observed that the DIO group had the highest lipid-phospholipid ratio. In sperm membrane protein profile analysis, it was found that both additives exerted protective effects at different levels. The highest total protein content was seen in the DIO 4 mM and NAR 4 mM groups. 8-hydroxydeoxyguanosine (8-OhDG) positivity was more common in the control group than in the DIO and NAR groups. Cu-Zn superoxide dismutase (SOD) expression was lower in the control group and more intense in all other groups. Positive results were especially observed in the acrosome of the sperm cells.<BR><B>CONCLUSIONS: </B>The addition of NAR and DIO to the ram semen extender increased the quality of sperm parameters after the freeze-thaw process. Doi.org/10.54680/fr24510110412.