{Reference Type}: Journal Article
{Title}: Screening of novel disease genes of sepsis-induced myocardial Disfunction by RNA sequencing and bioinformatics analysis.
{Author}: Yao H;Xiao Z;Liu S;Gao X;Wu Z;Li D;Yi Z;Zhou H;Zhang W;
{Journal}: Genomics
{Volume}: 116
{Issue}: 5
{Year}: 2024 Aug 5
{Factor}: 4.31
{DOI}: 10.1016/j.ygeno.2024.110911
{Abstract}: <B>BACKGROUND: </B>There is still a lack of effective treatment for sepsis-induced myocardial dysfunction (SIMD), while the pathogenesis of SIMD still remains largely unexplained.<BR><B>METHODS: </B>RNA sequencing results (GSE267388 and GSE79962) were used for cross-species integrative analysis. Bioinformatic analyses were used to delve into function, tissue- and cell- specificity, and interactions of genes. External datasets and qRT-PCR experiments were used for validation. L1000 FWD was used to predict targeted drugs, and 3D structure files were used for molecular docking.<BR><B>RESULTS: </B>Based on bioinformatic analyses, ten differentially expressed genes were selected as genes of interest, seven of which were verified to be significantly differential expression. Bucladesine was considered as a potential targeted drug for SIMD, which banded to seven target proteins primarily by forming hydrogen bonds.<BR><B>CONCLUSIONS: </B>It was considered that Cebpd, Timp1, Pnp, Osmr, Tgm2, Cp, and Asb2 were novel disease genes, while bucladesine was a potential therapeutic drug, of SIMD.