{Reference Type}: Journal Article
{Title}: The uncleaved signal peptide of Bombyx mori nucleopolyhedrovirus GP64 preferentially activates BmSpz7 expression, contributing to protein secretion.
{Author}: Hou Y;Zhang T;Yin K;Hao B;Huang J;
{Journal}: Biochem Biophys Res Commun
{Volume}: 736
{Issue}: 0
{Year}: 2024 Aug 3
{Factor}: 3.322
{DOI}: 10.1016/j.bbrc.2024.150499
{Abstract}: Bombyx mori nucleopolyhedrovirus (BmNPV) significantly impacts silkworm sericulture, causing substantial economic losses. The GP64 protein, a primary envelope protein of BmNPV budded virus (BV), retains its signal peptide (SP) in the mature form, crucial for its translocation to the plasma membrane (PM) and viral infectivity. This study investigates the role of the uncleaved SP of GP64 in activating the expression of BmSpz7, a novel Spätzle family member identified through RNA-seq analysis. We cloned and characterized BmSpz7, demonstrating its upregulated expression in BmN cells and silkworm larvae infected with BmNPV containing GP64 with an uncleaved SP. Additionally, transient expression of GP64's SP significantly enhanced BmSpz7 expression and protein secretion. These findings suggest that the uncleaved SP of GP64 plays a pivotal role in activating BmSpz7, providing new insights into the molecular interactions between BmNPV and its host, and revealing potential targets for antiviral strategies in sericulture.