{Reference Type}: Journal Article
{Title}: Synthesis and comparison of the performance of two different water-soluble phthalocyanine based electrochemical biosensor.
{Author}: Kocak M;Can Osmanogullari S;Soyler D;Arın Ozturmen B;Bekircan O;Biyiklioglu Z;Soylemez S;
{Journal}: Bioelectrochemistry
{Volume}: 160
{Issue}: 0
{Year}: 2024 Aug 2
{Factor}: 5.76
{DOI}: 10.1016/j.bioelechem.2024.108788
{Abstract}: Herein, a comparative study between novel water-soluble phthalocyanine-based biosensors was performed for the application of glucose sensing. For this purpose, two different copper (II) and manganese (III) phthalocyanines and their water-soluble derivatives were synthesized, and then their role as a supporting material for enzyme immobilization was evaluated by comparing their sensor performances. Two different phthalocyanine (AP-OH2-MnQ (MnPc) and AP-OH2-CuQ (CuPc)) were tested using electrochemical biosensor with immobilized glucose oxidase (GOx). To the best of our knowledge, the related water-soluble phthalocyanine-based glucose biosensors were attempted for the first time, and the developed approach resulted in improved biosensor characteristics. The constructed biosensors GE/MnPc/GOx and GE/CuPc/GOx showed good linearity between 0.003-1.0 mM and 0.05-0.4 mM, respectively. The limit of detection was estimated at 0.0026 mM for the GE/MnPc/GOx and 0.019 mM for the GE/CuPc/GOx. KMapp and sensitivity values were also calculated as 0.026 mM and 175.043 µAmM-1 cm-2 for the GE/MnPc/GOx biosensor and 0.178 mM and 117.478 µAmM-1 cm-2 for the GE/CuPc/GOx biosensor. Moreover, the fabricated biosensors were successfully tested to detect glucose levels in beverages with high recovery results. The present study shows that the proposed water-soluble phthalocyanines could be a good alternative for quick and cheap glucose sensing with improved analytical characteristics.