{Reference Type}: Journal Article {Title}: Cognitive workload estimation using physiological measures: a review. {Author}: Das Chakladar D;Roy PP; {Journal}: Cogn Neurodyn {Volume}: 18 {Issue}: 4 {Year}: 2024 Aug {Factor}: 3.473 {DOI}: 10.1007/s11571-023-10051-3 {Abstract}: Estimating cognitive workload levels is an emerging research topic in the cognitive neuroscience domain, as participants' performance is highly influenced by cognitive overload or underload results. Different physiological measures such as Electroencephalography (EEG), Functional Magnetic Resonance Imaging, Functional near-infrared spectroscopy, respiratory activity, and eye activity are efficiently used to estimate workload levels with the help of machine learning or deep learning techniques. Some reviews focus only on EEG-based workload estimation using machine learning classifiers or multimodal fusion of different physiological measures for workload estimation. However, a detailed analysis of all physiological measures for estimating cognitive workload levels still needs to be discovered. Thus, this survey highlights the in-depth analysis of all the physiological measures for assessing cognitive workload. This survey emphasizes the basics of cognitive workload, open-access datasets, the experimental paradigm of cognitive tasks, and different measures for estimating workload levels. Lastly, we emphasize the significant findings from this review and identify the open challenges. In addition, we also specify future scopes for researchers to overcome those challenges.