{Reference Type}: Journal Article
{Title}: Comprehensive analysis of single-nucleotide variants and alternative polyadenylation between inbred and outbred pigs.
{Author}: Han Y;Tu W;Zhang Y;Huang J;Meng X;Wu Q;Li S;Liu B;Michal JJ;Jiang Z;Tan Y;Zhou X;Wang H;
{Journal}: Int J Biol Macromol
{Volume}: 278
{Issue}: 0
{Year}: 2024 Aug 3
{Factor}: 8.025
{DOI}: 10.1016/j.ijbiomac.2024.134416
{Abstract}: Inbreeding can lead to the accumulation of homozygous single nucleotide polymorphisms (SNPs) in the genome, which can significantly affect gene expression and phenotype. In this study, we examined the impact of homozygous SNPs resulting from inbreeding on alternative polyadenylation (APA) site selection and the underlying genetic mechanisms using inbred Luchuan pigs. Genome resequencing revealed that inbreeding results in a high accumulation of homozygous SNPs within the pig genome. 3' mRNA-seq on leg muscle, submandibular lymph node, and liver tissues was performed to identify differences in APA events between inbred and outbred Luchuan pigs. We revealed different tissue-specific APA usage caused by inbreeding, which were associated with different biological processes. Furthermore, we explored the role of polyadenylation signal (PAS) SNPs in APA regulation under inbreeding and identified key genes such as PUM1, SCARF1, RIPOR2, C1D, and LRRK2 that are involved in biological processes regulation. This study provides resources and sheds light on the impact of genomic homozygosity on APA regulation, offering insights into genetic characteristics and biological processes associated with inbreeding.