{Reference Type}: Journal Article
{Title}: Transcriptomic balance and optimal growth are determined by cell size.
{Author}: Vidal PJ;Pérez AP;Yahya G;Aldea M;
{Journal}: Mol Cell
{Volume}: 84
{Issue}: 17
{Year}: 2024 Sep 5
{Factor}: 19.328
{DOI}: 10.1016/j.molcel.2024.07.005
{Abstract}: Cell size and growth are intimately related across the evolutionary scale, but whether cell size is important to attain maximal growth or fitness is still an open question. We show that growth rate is a non-monotonic function of cell volume, with maximal values around the critical size of wild-type yeast cells. The transcriptome of yeast and mouse cells undergoes a relative inversion in response to cell size, which we associate theoretically and experimentally with the necessary genome-wide diversity in RNA polymerase II affinity for promoters. Although highly expressed genes impose strong negative effects on fitness when the DNA/mass ratio is reduced, transcriptomic alterations mimicking the relative inversion by cell size strongly restrain cell growth. In all, our data indicate that cells set the critical size to obtain a properly balanced transcriptome and, as a result, maximize growth and fitness during proliferation.