{Reference Type}: Journal Article
{Title}: Expression of CD163 and major histocompatibility complex class I as diagnostic markers for idiopathic inflammatory myopathies.
{Author}: Ghang B;Nam SH;Choi W;Kim HJ;Lee J;Lim DH;Ahn SM;Oh JS;Hong S;Kim YG;Lee CK;Kim J;Yoo B;Nam SJ;
{Journal}: Arthritis Res Ther
{Volume}: 26
{Issue}: 1
{Year}: 2024 Jul 30
{Factor}: 5.606
{DOI}: 10.1186/s13075-024-03364-z
{Abstract}: BACKGROUND: To develop an inflammation-related immunohistochemistry marker-based algorithm that confers higher diagnostic ability for idiopathic inflammatory myopathies (IIMs) than IIM-related histopathologic features.
METHODS: Muscle biopsy tissues from 129 IIM patients who met the 2017 EULAR/ACR criteria and 73 control tissues from patients with non-inflammatory myopathies or healthy muscle specimens were evaluated for histological features and immunostaining results of CD3, CD4, CD8, CD20, CD68, CD163, MX1, MHC class I, MHC class II, and HLA-DR. Diagnostic algorithms for IIM were developed based on the results of the classification and regression tree (CART) analysis, which used immunostaining results as predictor variables for classifying patients with IIMs.
RESULTS: In the analysis set (IIM, n = 129; control, n = 73), IIM-related histopathologic features had a diagnostic accuracy of 87.6% (sensitivity 80.6%; specificity 100.0%) for IIMs. Notably, muscular expression of CD163 (99.2% vs. 20.8%, p < 0.001) and MHC class I (87.6% vs. 23.1%, p < 0.001) was significantly higher in the IIM group than in controls. Based on the CART analysis results, we developed an algorithm combining CD163 and MHC class I expression that conferred a diagnostic accuracy of 95.5% (sensitivity 96.1%; specificity 94.5%). In addition, our algorithm was able to correctly diagnose IIM in 94.1% (16/17) of patients who did not meet the 2017 EUALR/ACR criteria but were diagnosed as having IIMs by an expert physician.
CONCLUSIONS: Combination of CD163 and MHC class I muscular expression may be useful in diagnosing IIMs.