{Reference Type}: Journal Article
{Title}: The role of Pim-1 kinases in inflammatory signaling pathways.
{Author}: Baek HS;Kim N;Park JW;Kwon TK;Kim S;
{Journal}: Inflamm Res
{Volume}: 0
{Issue}: 0
{Year}: 2024 Jul 30
{Factor}: 6.986
{DOI}: 10.1007/s00011-024-01924-2
{Abstract}: <B>OBJECTIVE: </B>This observational study investigated the regulatory mechanism of Pim-1 in inflammatory signaling pathways.<BR><B>METHODS: </B>THP-1, RAW 264.7, BV2, and Jurkat human T cell lines were used.<BR><B>METHODS: </B>None.<BR><B>METHODS: </B>Lipopolysaccharide (LPS) was used to induce inflammation, followed by PIM1 knockdown. Western blot, immunoprecipitation, immunofluorescence, and RT-PCR assays were used to assess the effect of PIM1 knockdown on LPS-induced inflammation.<BR><B>RESULTS: </B>PIM1 knockdown in macrophage-like THP-1 cells suppressed LPS-induced upregulation of pro-inflammatory cytokines, inducible nitric oxide synthase, cyclooxygenase-2, phosphorylated Janus kinase, signal transducer and activator of transcription 3, extracellular signal-regulated kinase, c-Jun N-terminal kinase, p38, and nuclear factor kappa B p65 (NF-κB p65). It also suppressed upregulation of inhibitor of NF-κB kinase α/β and enhanced the nuclear translocation of NF-κB p65. Moreover, it inhibited the upregulation of Nod-like receptor family pyrin domain-containing 3 (NLRP3) and cleavage of caspase-1 induced by co-treatment of LPS with adenosine triphosphate. Additionally, p-transforming growth factor-β-activated kinase 1 (TAK1) interacted with Pim-1. All three members of Pim kinases (Pim-1, Pim-2, and Pim-3) were required for LPS-mediated inflammation in macrophages; however, unlike Pim-1 and Pim-3, Pim-2 functioned as a negative regulator of T cell activity.<BR><B>CONCLUSIONS: </B>Pim-1 interacts with TAK1 in LPS-induced inflammatory responses and is involved in MAPK/NF-κB/NLRP3 signaling pathways. Additionally, considering the negative regulatory role of Pim-2 in T cells, further in-depth studies on their respective functions are needed.