{Reference Type}: Journal Article
{Title}: Non-Faradaic Impedimetric Detection of Heavy Metal Ions via a Hybrid Nanoparticle-DNAzyme Biosensor.
{Author}: Panagopoulou C;Skotadis E;Aslanidis E;Tzourmana G;Rapesi A;Tsioustas C;Kainourgiaki M;Kleitsiotis G;Tsekenis G;Tsoukalas D;
{Journal}: Biosensors (Basel)
{Volume}: 14
{Issue}: 7
{Year}: 2024 Jun 27
{Factor}: 5.743
{DOI}: 10.3390/bios14070321
{Abstract}: Due to rapid industrialization, novel water-quality monitoring techniques for the detection of highly toxic and hazardous heavy metal ions are essential. Herein, a hybrid noble nanoparticle/DNAzyme electrochemical biosensor is proposed for the simultaneous and label-free detection of Pb2+ and Cr3+ in aqueous solutions. The sensor is based on the combination of a two-dimensional naked-platinum nanoparticle film and DNAzymes, whose double-helix configuration disassembles into smaller fragments in the presence of target-specific heavy metal ions. The electrochemical behavior of the fabricated sensor was investigated with non-faradaic electrochemical impedance spectroscopy (EIS), resulting in the successful detection of Pb2+ and Cr3+ well below their maximum permitted levels in tap water. So far, there has been no report on the successful detection of heavy metal ions utilizing the non-faradaic electrochemical impedance spectroscopy technique based on advanced nanomaterials paired with DNAzymes. This is also one of the few reports on the successful detection of chromium (III) via a sensor incorporating DNAzymes.