{Reference Type}: Journal Article
{Title}: Use of Single-Domain Antibodies Against the NSm Protein for the Detection of Cells Infected by Rift Valley Fever Virus.
{Author}: Romanet C;Tamietti C;Mériaux V;Bontems F;Montagutelli X;Lafaye P;Flamand M;
{Journal}: Methods Mol Biol
{Volume}: 2824
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3926-9_11
{Abstract}: Single-domain antibodies, referred to as VHH (variable heavy chains of heavy chain-only antibodies) or in their commercial name as nanobodies, are potent tools for the detection of target proteins in biological samples. They have the advantage of being highly stable, specific, and sensitive, with affinities reaching the nanomolar range. We utilized this tool to develop a rapid detection method that discriminates cells infected with Rift Valley fever virus (RVFV), based on the intracellular detection of the viral nonstructural NSm protein localized on the outer membrane of mitochondria. Here we describe how NSm-specific VHHs have been produced, cloned, and characterized, highlighting their value in RVFV research and diagnosis. This work may also raise interest in other potential applications such as antiviral therapy.