{Reference Type}: Journal Article
{Title}: A spatiotemporal molecular atlas of mouse spinal cord injury identifies a distinct astrocyte subpopulation and therapeutic potential of IGFBP2.
{Author}: Wang Z;Li Z;Luan T;Cui G;Shu S;Liang Y;Zhang K;Xiao J;Yu W;Cui J;Li A;Peng G;Fang Y;
{Journal}: Dev Cell
{Volume}: 0
{Issue}: 0
{Year}: 2024 Jul 16
{Factor}: 13.417
{DOI}: 10.1016/j.devcel.2024.06.016
{Abstract}: Spinal cord injury (SCI) triggers a cascade of intricate molecular and cellular changes that determine the outcome. In this study, we resolve the spatiotemporal organization of the injured mouse spinal cord and quantitatively assess in situ cell-cell communication following SCI. By analyzing existing single-cell RNA sequencing datasets alongside our spatial data, we delineate a subpopulation of Igfbp2-expressing astrocytes that migrate from the white matter (WM) to gray matter (GM) and become reactive upon SCI, termed Astro-GMii. Further, Igfbp2 upregulation promotes astrocyte migration, proliferation, and reactivity, and the secreted IGFBP2 protein fosters neurite outgrowth. Finally, we show that IGFBP2 significantly reduces neuronal loss and remarkably improves the functional recovery in a mouse model of SCI in vivo. Together, this study not only provides a comprehensive molecular atlas of SCI but also exemplifies how this rich resource can be applied to endow cells and genes with functional insight and therapeutic potential.