{Reference Type}: Journal Article
{Title}: Tandem repeats in the genome of Toxoplasma gondii display compositional bias that impacts in protein structure.
{Author}: Hjelt V;Goldman A;Martin V;Ruybal P;Moretta R;
{Journal}: Gene
{Volume}: 928
{Issue}: 0
{Year}: 2024 Nov 30
{Factor}: 3.913
{DOI}: 10.1016/j.gene.2024.148774
{Abstract}: Repetitive elements in DNA sequences are a hallmark of Apicomplexan protozoa. A genome-wide screening for Tandem Repeats was conducted in Toxoplasma gondii and related Coccidian parasites with a novel strategy to assess compositional bias. A conserved pattern of GC skew and purine-pyrimidine bias was observed. Compositional bias was also present at the protein level. Glutamic acid was the most abundant amino acid in the purine (GA) rich cluster, while Serine prevailed in pyrimidine (CT) rich cluster. Purine rich repeats, and consequently glutamic acid abundance, correlated with high scores for intrinsically disordered protein regions/domains. Finally, variability was established for repetitive regions within a well-known rhoptry antigen (ROP1) and an uncharacterized hypothetical protein with similar features. The approach we present could be useful to identify potential antigens bearing repetitive elements.