{Reference Type}: Journal Article
{Title}: Using Ex Vivo Tonsil Organoids to Study Memory B Cells.
{Author}: Wagoner ZW;Mitul MT;Wagar LE;
{Journal}: Methods Mol Biol
{Volume}: 2826
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3950-4_1
{Abstract}: Tools to study memory B cell (MBC) development and function are needed to understand their role in supporting sustained protection against recurrent infections. While human MBCs are traditionally measured using blood, there is a growing interest in elucidating their behavior within lymphoid tissues, which are the main sites where adaptive immune responses are orchestrated. In this chapter, we introduce a high-throughput organoid system that is derived from primary human lymphoid tissues. The approach can recapitulate many hallmarks of successful adaptive immune responses and capture inter-individual variation in response to a variety of stimuli. Lymphoid tissue organoids enable characterization of pre-existing antigen-specific MBCs within an entirely human system and can provide valuable insights into MBC dynamics.