{Reference Type}: Journal Article
{Title}: Development of an Integrated Disposable Device for SARS-CoV-2 Nucleic Acid Extraction and Detection.
{Author}: Ma J;Hao YZ;Hou ML;Zhang XS;Liu JD;Meng HD;Chang JB;Ma XJ;Liu JH;Ying QJ;Wang XH;Li HX;Cao YX;Zhang XG;
{Journal}: Biomed Environ Sci
{Volume}: 37
{Issue}: 6
{Year}: 2024 Jun 20
{Factor}: 2.831
{DOI}: 10.3967/bes2024.070
{Abstract}: <B>UNASSIGNED: </B>To develop a highly sensitive and rapid nucleic acid detection method for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).<BR><B>UNASSIGNED: </B>We designed, developed, and manufactured an integrated disposable device for SARS-CoV-2 nucleic acid extraction and detection. The precision of the liquid transfer and temperature control was tested. A comparison between our device and a commercial kit for SARS-Cov-2 nucleic acid extraction was performed using real-time fluorescence reverse transcription polymerase chain reaction (RT-PCR). The entire process, from SARS-CoV-2 nucleic acid extraction to amplification, was evaluated.<BR><B>UNASSIGNED: </B>The precision of the syringe transfer volume was 19.2 ± 1.9 μL (set value was 20), 32.2 ± 1.6 (set value was 30), and 57.2 ± 3.5 (set value was 60). Temperature control in the amplification tube was measured at 60.0 ± 0.0 °C (set value was 60) and 95.1 ± 0.2 °C (set value was 95) respectively. SARS-Cov-2 nucleic acid extraction yield through the device was 7.10 × 10 6 copies/mL, while a commercial kit yielded 2.98 × 10 6 copies/mL. The mean time to complete the entire assay, from SARS-CoV-2 nucleic acid extraction to amplification detection, was 36 min and 45 s. The detection limit for SARS-CoV-2 nucleic acid was 250 copies/mL.<BR><B>UNASSIGNED: </B>The integrated disposable devices may be used for SARS-CoV-2 Point-of-Care test (POCT).