{Reference Type}: Journal Article
{Title}: Anther Culture Protocols for Barley and Wheat.
{Author}: Broughton S;Castello M;Liu L;Killen J;McMullan C;
{Journal}: Methods Mol Biol
{Volume}: 2827
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3954-2_17
{Abstract}: Doubled haploid (DH) techniques remain valuable tools for wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) genetic improvement, and DH populations are used extensively in breeding and research endeavors. Several techniques are available for DH production in wheat and barley. Here, we describe two simple, robust anther culture methods used to produce more than 15,000 DH wheat and barley lines annually in Australia.