{Reference Type}: Journal Article {Title}: Generating controlled gust perturbations using vortex rings. {Author}: Gupta D;Sane SP;Arakeri JH; {Journal}: PLoS One {Volume}: 19 {Issue}: 7 {Year}: 2024 {Factor}: 3.752 {DOI}: 10.1371/journal.pone.0305084 {Abstract}: To understand the locomotory mechanisms of flying and swimming animals, it is often necessary to develop assays that enable us to measure their responses to external gust perturbations. Typically, such measurements have been carried out using a variety of gusts which are difficult to control or characterize owing to their inherently turbulent nature. Here, we present a method of generating discrete gusts under controlled laboratory conditions in the form of a vortex rings which are well-characterized and highly controllable. We also provide the theoretical guidelines underlying the design of gust generators for specific applications. As a case study, we tested the efficacy of this method to study the flight response of freely-flying soldier flies Hermetia illucens. The vortex ring based method can be used to generate controlled gusts to study diverse phenomena ranging from a natural flight in insects to the artificial flight of insect-sized drones and micro-aerial vehicles.