{Reference Type}: Journal Article
{Title}: Dual-specificity phosphatases 13 and 27 as key switches in muscle stem cell transition from proliferation to differentiation.
{Author}: Hayashi T;Sadaki S;Tsuji R;Okada R;Fuseya S;Kanai M;Nakamura A;Okamura Y;Muratani M;Wenchao G;Sugasawa T;Mizuno S;Warabi E;Kudo T;Takahashi S;Fujita R;
{Journal}: Stem Cells
{Volume}: 42
{Issue}: 9
{Year}: 2024 Sep 10
{Factor}: 5.845
{DOI}: 10.1093/stmcls/sxae045
{Abstract}: Muscle regeneration depends on muscle stem cell (MuSC) activity. Myogenic regulatory factors, including myoblast determination protein 1 (MyoD), regulate the fate transition of MuSCs. However, the direct target of MYOD in the process is not completely clear. Using previously established MyoD knock-in (MyoD-KI) mice, we revealed that MyoD targets dual-specificity phosphatase (Dusp) 13 and Dusp27. In Dusp13:Dusp27 double knock-out mice, the ability for muscle regeneration after injury was reduced. Moreover, single-cell RNA sequencing of MyoD-high expressing MuSCs from MyoD-KI mice revealed that Dusp13 and Dusp27 are expressed only in specific populations within MyoD-high MuSCs, which also express Myogenin. Overexpressing Dusp13 in MuSCs causes premature muscle differentiation. Thus, we propose a model where DUSP13 and DUSP27 contribute to the fate transition of MuSCs from proliferation to differentiation during myogenesis.