{Reference Type}: Journal Article
{Title}: An outer membrane vesicle specific lipoprotein promotes Porphyromonas gingivalis aggregation on red blood cells.
{Author}: Rothenberger CM;Yu M;Kim HM;Cheung YW;Chang YW;Davey ME;
{Journal}: Curr Res Microb Sci
{Volume}: 7
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1016/j.crmicr.2024.100249
{Abstract}: Porphyromonas gingivalis uses a variety of mechanisms to actively interact with and promote the hydrolysis of red blood cells (RBCs) to obtain iron in the form of heme. In this study, we investigated the function of lipoprotein PG1881 which was previously shown to be up-regulated during subsurface growth and selectively enriched on outer membrane vesicles (OMVs). Our results show that wildtype strain W83 formed large aggregates encompassing RBCs whereas the PG1881 deletion mutant remained predominately as individual cells. Using a PG1881 antibody, immunofluorescence revealed that the wildtype strain's aggregation to RBCs involves an extracellular matrix enriched with PG1881. Our findings discover that RBCs elicit cell aggregation and matrix formation by P. gingivalis and that this process is promoted by an OMV-specific lipoprotein. We propose this strategy is advantageous for nutrient acquisition as well as dissemination from the oral cavity and survival of this periodontal pathogen.