{Reference Type}: Journal Article {Title}: Improving bee feed recipes to safeguard honeybee colonies during times of food scarcity. {Author}: Wang X;Zheng X;Guo N;Geng M;Wang R;Huang T;Ji Q;Liu Z;Zhao Y; {Journal}: Arch Insect Biochem Physiol {Volume}: 116 {Issue}: 3 {Year}: 2024 Jul {Factor}: 2.454 {DOI}: 10.1002/arch.22129 {Abstract}: In beekeeping, when natural nectar or pollen sources become limited, it is crucial to provide supplemental bee feed to maintain the viability of the bee colony. This study was conducted during the autumn food shortage season, during which bees were fed with different proportions of modified bee feed. We identified an optimal bee diet by evaluating honeybee longevity, food consumption, body weight, and gut microbe distribution, with natural pollen serving as a control diet. The results indicated that bees preferred a mixture of 65% defatted soy flour, 20% corn protein powder, 13% wheat germ flour, 2% yeast powder, and a 50% sucrose solution. This bee food recipe significantly increased the longevity, feed consumption, and body weight of bees. The group fed the natural pollen diet exhibited a greater abundance of essential intestinal bacteria. The bee diets used in this study contained higher protein levels and lower concentrations of unsaturated fatty acids and vitamins than did the diets stored within the colonies. Therefore, we propose that incorporating both bee feed and natural pollen in beekeeping practices will achieve more balanced nutritional intake.