{Reference Type}: Journal Article
{Title}: TIR domains of TLR family-from the cell culture to the protein sample for structural studies.
{Author}: Lushpa VA;Goncharuk MV;Talyzina IA;Arseniev AS;Bocharov EV;Mineev KS;Goncharuk SA;
{Journal}: PLoS One
{Volume}: 19
{Issue}: 7
{Year}: 2024
{Factor}: 3.752
{DOI}: 10.1371/journal.pone.0304997
{Abstract}: Toll-like receptors (TLRs) are key players in the innate immune system. Despite the great efforts in TLR structural biology, today we know the spatial structures of only four human TLR intracellular TIR domains. All of them belong to one of five subfamilies of receptors. One of the main bottlenecks is the high-level production of correctly folded proteins in soluble form. Here we used a rational approach to find the optimal parameters to produce TIR domains of all ten human TLR family members in soluble form in E. coli cells. We showed that dozens of milligrams of soluble His-tagged TLR2/3/6/7TIR and MBP-tagged TLR3/5/7/8TIR can be produced. We also developed the purification protocols and demonstrated by CD and NMR spectroscopy that purified TLR2/3/7TIR demonstrate a structural organization inherent to TIR domains. This illustrates the correct folding of produced proteins and their suitability for further structural and functional investigations.