{Reference Type}: Journal Article
{Title}: NOX4-mediated astrocyte ferroptosis in Alzheimer's disease.
{Author}: Maimaiti Y;Su T;Zhang Z;Ma L;Zhang Y;Xu H;
{Journal}: Cell Biosci
{Volume}: 14
{Issue}: 1
{Year}: 2024 Jul 2
{Factor}: 9.584
{DOI}: 10.1186/s13578-024-01266-w
{Abstract}: This study investigates NADPH oxidase 4 (NOX4) involvement in iron-mediated astrocyte cell death in Alzheimer's Disease (AD) using single-cell sequencing data and transcriptomes. We analyzed AD single-cell RNA sequencing data, identified astrocyte marker genes, and explored biological processes in astrocytes. We integrated AD-related chip data with ferroptosis-related genes, highlighting NOX4. We validated NOX4's role in ferroptosis and AD in vitro and in vivo. Astrocyte marker genes were enriched in AD, emphasizing their role. NOX4 emerged as a crucial player in astrocytic ferroptosis in AD. Silencing NOX4 mitigated ferroptosis, improved cognition, reduced Aβ and p-Tau levels, and alleviated mitochondrial abnormalities. NOX4 promotes astrocytic ferroptosis, underscoring its significance in AD progression.