{Reference Type}: Journal Article
{Title}: Revitalizing gut barrier integrity: miR-192-5p's role in enhancing autophagy via Rictor in enteritis.
{Author}: Qiu P;Zhou K;Wang Y;Chen X;Xiao C;Li W;Chen Y;Chang Y;Liu J;Zhou F;Wang X;Shang J;Liu L;Zhao Q;
{Journal}: Am J Physiol Gastrointest Liver Physiol
{Volume}: 0
{Issue}: 0
{Year}: 2024 Jul 2
{Factor}: 4.871
{DOI}: 10.1152/ajpgi.00291.2023
{Abstract}: <B>BACKGROUND: </B>Intestinal inflammation and compromised barrier function are critical factors in the pathogenesis of gastrointestinal disorders. This study aimed to investigate the role of miR-192-5p in modulating intestinal epithelial barrier (IEB) integrity and its association with autophagy.<BR><B>METHODS: </B>A DSS-induced colitis model was used to assess the effects of miR-192-5p on intestinal inflammation. In vitro experiments involved cell culture and transient transfection techniques. Various assays, including dual-luciferase reporter gene assays, quantitative real-time PCR, western blotting, and measurements of transepithelial electrical resistance, were performed to evaluate changes in miR-192-5p expression, Rictor levels, and autophagy flux. Immunofluorescence staining, H&E staining, TEER measurements, and FITC-dextran analysis were also employed.<BR><B>RESULTS: </B>Our findings revealed a reduced expression of miR-192-5p in inflamed intestinal tissues, correlating with impaired IEB function. Overexpression of miR-192-5p alleviated TNF-induced IEB dysfunction by targeting Rictor, resulting in enhanced autophagy flux in enterocytes (ECs). Moreover, the therapeutic potential of miR-192-5p was substantiated in colitis mice, wherein increased miR-192-5p expression ameliorated intestinal inflammatory injury by enhancing autophagy flux in ECs through the modulation of Rictor.<BR><B>CONCLUSIONS: </B>Our study highlights the therapeutic potential of miR-192-5p in enteritis by demonstrating its role in regulating autophagy and preserving IEB function. Targeting the miR-192-5p/Rictor axis is a promising approach for mitigating gut inflammatory injury and improving barrier integrity in enteritis patients.