{Reference Type}: Journal Article
{Title}: Recombinant Protein Production in Suspension Mammalian Cells Using the BacMam Baculovirus Expression System.
{Author}: Puente-Massaguer E;Gòdia F;
{Journal}: Methods Mol Biol
{Volume}: 2829
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3961-0_25
{Abstract}: Mammalian cell lines are one of the best options when it comes to the production of complex proteins requiring specific glycosylation patterns. Plasmid DNA transfection and stable cell lines are frequently used for recombinant protein production, but they are expensive at large scale or can become time-consuming, respectively. The BacMam baculovirus (BV) is a safe and cost-effective platform to produce recombinant proteins in mammalian cells. The process of generating BacMam BVs is straightforward and similar to the generation of "insect" BVs, with different commercially available platforms. Although there are several protocols that describe recombinant protein expression with the BacMam BV in adherent cell lines, limited information is available on suspension cells. Therefore, it is of relevance to define the conditions to produce recombinant proteins in suspension cell cultures with BacMam BVs that facilitate bioprocess transfer to larger volumes. Here, we describe a method to generate a high titer BacMam BV stock and produce recombinant proteins in suspension HEK293 cells.