{Reference Type}: Journal Article
{Title}: Recombinant AAV Purification.
{Author}: Joshi PRH;Venereo-Sanchez A;
{Journal}: Methods Mol Biol
{Volume}: 2829
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3961-0_15
{Abstract}: Purification of rAAV is a crucial unit operation of the AAV production process. It enables the capture of AAV and removal of contaminants such as host cell proteins, host cell DNA, and other cell culture-related impurities. Here we describe the purification of rAAV produced in insect cells Sf9/rBEV by immuno-affinity capture chromatography. The method is fully scale-amenable unlike other traditional purification methods based on ultracentrifugation. The method reported herein has two main steps: (1) the clarification of cell lysate by depth filtration and (2) the selective capture and single-step purification of AAV via immune-affinity chromatography. This purification method has been successfully implemented to purify the majority of wild-type AAV serotypes.