{Reference Type}: Journal Article {Title}: Handling and Tagging Techniques for Implanting Juvenile American Shad with a New Acoustic Microtransmitter. {Author}: Deters KA;Janak JM;Mueller RP;Boehnke BT;Deng ZD; {Journal}: J Vis Exp {Volume}: 0 {Issue}: 208 {Year}: 2024 Jun 14 {Factor}: 1.424 {DOI}: 10.3791/65694 {Abstract}: The use of telemetry techniques to better understand the behavior and survival of juvenile American shad (Alosa sapidissima), as they migrate through hydropower systems, has been challenging because shad are widely known to be particularly sensitive to handling. The goal of this study was to develop a tagging protocol using a new, acoustic micro transmitter that minimizes the detrimental effects of the tagging process and maximizes post-tagging survival of juvenile American shad. Limiting out-of-water handling and the use of brackish saltwater (7.5 parts per thousand) before and after tagging improved survival for shad tagged using a simple pectoral implantation method. This protocol provides a detailed, step-by-step procedure for tagging juvenile shad with acoustic transmitters. Fish tagged using this procedure and held in the laboratory for 60 days had an 81.5% survival rate, compared to 70% for their untagged counterparts. The successful tagging and handling practices developed in this study could be applied to field telemetry studies of juvenile shad and other sensitive species.