{Reference Type}: Journal Article
{Title}: Role of the gut microbiota in cefoperazone/sulbactam-induced epilepsy in mice with chronic renal failure.
{Author}: Wu Y;Gu D;Li J;Li J;Hou G;
{Journal}: Ren Fail
{Volume}: 46
{Issue}: 2
{Year}: 2024 Dec
{Factor}: 3.222
{DOI}: 10.1080/0886022X.2024.2371551
{Abstract}: <B>UNASSIGNED: </B>The mechanism of cefoperazone/sulbactam-induced epilepsy in chronic kidney disease (CKD) patients is not yet clear. We hypothesized that cefoperazone/sulbactam-induced epilepsy could be based on two main factors: neurotoxicity caused by drug accumulation after renal failure and an abnormal gut microbiota (GM).<BR><B>UNASSIGNED: </B>A chronic renal failure (CRF) model in mice was established, and then different doses of cefoperazone/sulbactam were injected to induce epilepsy in mice. Normal mouse feces for fecal microbiota transplantation (FMT) were collected. We observed the changes in feces, mental state, and activity of each group of mice. After killing, we collected kidneys and colon for H&E staining. We collected mouse feces for the 16S RNA sequencing of bacteria.<BR><B>UNASSIGNED: </B>All CRF mice injected with different concentrations of cefoperazone/sulbactam experienced grade-V seizures and eventually died, whereas normal control mice did not. However, after FMT intervention, the time of epilepsy onset and death in mice was delayed. Early FMT intervention resulted in more mice surviving (p = .0359). Moreover, the villi in the mucosal of group-CS layer fell off, goblet cells missed, and crypts disappeared. The mucosal layer and submucosa were clearly separated. The morphology of intestinal tissue of the CFS and FS group was improved. After FMT, the changes of the GM were observed.<BR><B>UNASSIGNED: </B>The GM may be involved in the epilepsy induced by cefoperazone/sulbactam in CRF mice. FMT can delay the onset of epilepsy in CRF mice induced by cefoperazone/sulbactam, and the earlier the intervention, the better the effect.