{Reference Type}: Journal Article
{Title}: Immunosuppressive alkaloids from Narcissus tazetta subsp. Chinensis and the mechanism of (+)-narciclasine in vitro and in vivo.
{Author}: Wang WL;Wu XY;Luo XY;Tang YQ;Cui J;Huang XY;Jiang YC;Liu Y;Li LM;
{Journal}: Phytochemistry
{Volume}: 225
{Issue}: 0
{Year}: 2024 Sep 25
{Factor}: 4.004
{DOI}: 10.1016/j.phytochem.2024.114198
{Abstract}: Three previously undescribed and sixteen known alkaloids were bioguidedly isolated from the bulbs of Narcissus tazetta subsp. chinensis (M.Roem.) Masamura & Yanagih. The structures were elucidated by spectroscopic data, including HRESIMS, NMR, and ECD. Eleven of the isolated alkaloids exhibited immunosuppressive activity on the proliferation of human T cells. (+)-Narciclasine (18) showed the most significantly suppressive activity with an IC50 value of 14 ± 5 nM. In vitro, (+)-narciclasine (18) blocked NF-κB signal transduction, but did not affect PI3K/AKT signal transduction. What was more, (+)-narciclasine significantly reduced ALT and AST levels and alleviated liver damage induced by ConA in AIH mouse model.