{Reference Type}: Journal Article {Title}: A study of the effect of testing for diversification of healthcare waste sterilization and crushing facilities. {Author}: Park YS;Kim MJ;Yoon YS;Jeon TW;Choi HH; {Journal}: Heliyon {Volume}: 10 {Issue}: 11 {Year}: 2024 Jun 15 {Factor}: 3.776 {DOI}: 10.1016/j.heliyon.2024.e31962 {Abstract}: This study conducted sterilization testing under different conditions using different strains for sterilization and crushing, the intermediate healthcare waste treatment phase, and proposed strategies for diversifying corresponding facilities in addition to promoting their installation. Five indicator microorganisms were selected to test the sterilization efficiency of steam, microwave, and chemical methods. Steam sterilization testing was conducted in accordance with legal and technological standards, microwave testing was carried out according to the legal standard, and chemical sterilization employed three typical compounds. Steam and microwave sterilization achieved 99.9999 % inactivation rates for all five strains under both conditions used; whereas under the chemical sterilization analyses, sodium hypochlorite (1000 ppm) failed to meet the inactivation requirement of the fungal strain Candida albicans, requiring further investigation. Based on these findings, this study presents strategies for diversifying sterilization·crushing facilities and promoting their installation.