{Reference Type}: Journal Article
{Title}: PC Gene Affects Milk Production Traits in Dairy Cattle.
{Author}: Du A;Guo Z;Chen A;Xu L;Sun D;Han B;
{Journal}: Genes (Basel)
{Volume}: 15
{Issue}: 6
{Year}: 2024 May 29
{Factor}: 4.141
{DOI}: 10.3390/genes15060708
{Abstract}: In previous work, we found that PC was differentially expressed in cows at different lactation stages. Thus, we deemed that PC may be a candidate gene affecting milk production traits in dairy cattle. In this study, we found the polymorphisms of PC by resequencing and verified their genetic associations with milk production traits by using an animal model in a cattle population. In total, we detected six single-nucleotide polymorphisms (SNPs) in PC. The single marker association analysis showed that all SNPs were significantly associated with the five milk production traits (p < 0.05). Additionally, we predicted that allele G of 29:g.44965658 in the 5' regulatory region created binding sites for TF GATA1 and verified that this allele inhibited the transcriptional activity of PC by the dual-luciferase reporter assay. In conclusion, we proved that PC had a prominent genetic effect on milk production traits, and six SNPs with prominent genetic effects could be used as markers for genomic selection (GS) in dairy cattle, which is beneficial for accelerating the improvement in milk yield and quality in Chinese Holstein cows.