{Reference Type}: Journal Article
{Title}: SIMOA Diagnostics on Alzheimer's Disease and Frontotemporal Dementia.
{Author}: Chatziefstathiou A;Canaslan S;Kanata E;Vekrellis K;Constantinides VC;Paraskevas GP;Kapaki E;Schmitz M;Zerr I;Xanthopoulos K;Sklaviadis T;Dafou D;
{Journal}: Biomedicines
{Volume}: 12
{Issue}: 6
{Year}: 2024 Jun 4
{Factor}: 4.757
{DOI}: 10.3390/biomedicines12061253
{Abstract}: <B>BACKGROUND: </B>Accurate diagnosis of Alzheimer's disease (AD) and frontotemporal dementia (FTD) represents a health issue due to the absence of disease traits. We assessed the performance of a SIMOA panel in cerebrospinal fluid (CSF) from 43 AD and 33 FTD patients with 60 matching Control subjects in combination with demographic-clinical characteristics.<BR><B>METHODS: </B>136 subjects (AD: n = 43, FTD: n = 33, Controls: n = 60) participated. Single-molecule array (SIMOA), glial fibrillary acidic protein (GFAP), neurofilament light (NfL), TAU, and ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) in CSF were analyzed with a multiplex neuro 4plex kit. Receiver operating characteristic (ROC) curve analysis compared area under the curve (AUC), while the principal of the sparse partial least squares discriminant analysis (sPLS-DA) was used with the intent to strengthen the identification of confident disease clusters.<BR><B>RESULTS: </B>CSF exhibited increased levels of all SIMOA biomarkers in AD compared to Controls (AUCs: 0.71, 0.86, 0.92, and 0.94, respectively). Similar patterns were observed in FTD with NfL, TAU, and UCH-L1 (AUCs: 0.85, 0.72, and 0.91). sPLS-DA revealed two components explaining 19% and 9% of dataset variation.<BR><B>CONCLUSIONS: </B>CSF data provide high diagnostic accuracy among AD, FTD, and Control discrimination. Subgroups of demographic-clinical characteristics and biomarker concentration highlighted the potential of combining different kinds of data for successful and more efficient cohort clustering.