{Reference Type}: English Abstract
{Title}: [Intervention Effect and Mechanism of Regulating MiR-155 on Young Rats with Dysfunction of Blood Coagulation].
{Author}: Zhang YJ;Yuan EW;Qu HX;Guo HX;
{Journal}: Zhongguo Shi Yan Xue Ye Xue Za Zhi
{Volume}: 32
{Issue}: 3
{Year}: 2024 Jun
暂无{DOI}: 10.19746/j.cnki.issn.1009-2137.2024.03.031
{Abstract}: <B>OBJECTIVE: </B>To investigate the intervention effect and mechanism of regulating miR-155 on young rats with dysfunction of blood coagulation.<BR><B>METHODS: </B>Twenty-six healthy and clean SD male rats were selected to establish the coagulopathy models. Twenty-four rats successfully established models and were randomly divided into three groups: model group, up-regulated miR-155 group and down-regulated miR-155 group, with 8 rats in each group. The expression of miR-155 was detected by real-time fluorescence quantitative polymerase chain reaction. The changes of coagulation factors and coagulation indicators were observed. Liver pathological tissues were observed by HE staining. The expressions of HMGB1-RAGE/TLRs-NF-κB signaling pathway related proteins were detected by Western blot.<BR><B>RESULTS: </B>Compared with model group, the expressions of HMGB1, RAGE, TLR2, TLR4 and NF-κB were significantly increased in up-regulated miR-155 group (all P < 0.05), while decreased in down-regulated miR-155 group (all P < 0.05). Compared with model group, the expressions of coagulation factor Ⅱ, Ⅶ, Ⅸ, and Ⅹ were significantly decreased in up-regulated miR-155 group (all P < 0.05), while increased in down-regulated miR-155 group (P < 0.05). There was no significant difference in the expression of coagulation factor Ⅺ among the three groups (P >0.05). Compared with model group, the levels of prothrombin time (PT) and activated partial thromboplastin time (APTT) were lower and fibrinogen (FIB) was higher in up-regulated miR-155 group (all P < 0.05), while in the down-regulated miR-155 group they were opposite.<BR><B>CONCLUSIONS: </B>Down-regulation of miR-155 can effectively improve coagulation factors and coagulation indexes and inhibit inflammation in young rats with dysfunction of blood coagulopathy, and the mechanism may be related to HMGB1-RAGE/TLRs-NF-κB signaling pathway.<BR><B>UNASSIGNED: </B>调控miR-155对凝血功能障碍模型幼鼠的干预效果及作用机制研究.<BR><B>UNASSIGNED: </B>研究调控miR-155对凝血功能障碍模型幼鼠的干预效果及作用机制。.<BR><B>UNASSIGNED: </B>选取健康、清洁级SD雄性幼鼠26只，建立凝血功能障碍模型，成功24只随机分为模型、上调miR-155和下调miR-155共3组，每组各8只。实时荧光定量聚合酶链式反应检测各组幼鼠miR-155表达，观察各组幼鼠凝血因子水平、凝血指标变化，HE染色观察肝脏病理组织，Western blot法检测HMGB1-RAGE/TLRs-NF-κB信号通路相关蛋白表达。.<BR><B>UNASSIGNED: </B>与模型组比，上调miR-155组HMGB1、RAGE、TLR2、TLR4、NF-κB均明显增高（均P <0.05），而下调miR-155组表达均明显降低（均P <0.05）。与模型组比，上调miR-155组凝血因子Ⅱ、Ⅶ、Ⅸ、Ⅹ表达均明显降低（均P <0.05），而下调miR-155组均表达升高（均P <0.05）。三组凝血因子Ⅺ表达差异比较无统计学意义（P >0.05）。与模型组比，上调miR-155组凝血酶原时间、活化部分凝血活酶水平较低，纤维蛋白原水平较高（均P <0.05），而下调miR-155组正好相反。.<BR><B>UNASSIGNED: </B>下调miR-155能有效改善凝血功能障碍幼鼠凝血因子水平及凝血指标，抑制炎症反应，其作用机制可能与HMGB1-RAGE/TLRs-NF-κB信号通路有关。.