{Reference Type}: Journal Article
{Title}: The PDZ domain of the E protein in SARS-CoV induces carcinogenesis and poor prognosis in LUAD.
{Author}: Li S;Wang J;Dai X;Li C;Li T;Chen L;
{Journal}: Microbes Infect
{Volume}: 0
{Issue}: 0
{Year}: 2024 Jun 22
{Factor}: 9.57
{DOI}: 10.1016/j.micinf.2024.105381
{Abstract}: <B>BACKGROUND: </B>In both lung adenocarcinoma (LUAD) and severe acute respiratory syndrome (SARS), uncontrolled inflammation can be detected in lung tissue. The PDZ-binding motif (PBM) in the SARS-CoV-1 E protein has been demonstrated to be a virulence factor that induces a cytokine storm.<BR><B>METHODS: </B>To identify gene expression fluctuations induced by PBM, microarray sequencing data of lung tissue infected with wild-type (SARS-CoV-1-E-wt) or recombinant virus (SARS-CoV-1-E-mutPBM) were analyzed, followed by functional enrichment analysis. To understand the role of the screened genes in LUAD, overall survival and immune correlation were calculated.<BR><B>RESULTS: </B>A total of 12 genes might participate in the initial and developmental stages of LUAD through expression variation and mutation. Moreover, dysregulation of a total of 12 genes could lead to a poorer prognosis. In addition, the downregulation of MAMDC2 and ITGA8 by PBM could also affect patient prognosis. Although the conserved PBM (-D-L-L-V-) can be found at the end of the carboxyl terminus in multiple E proteins of coronaviruses, the specific function of each protein depends on the entire amino acid sequence.<BR><B>CONCLUSIONS: </B>In summary, PBM containing the SARS-CoV-1 E protein promoted the carcinogenesis of LUAD by dysregulating important gene expression profiles and subsequently influencing the immune response and overall prognosis.