{Reference Type}: Journal Article
{Title}: Involvement of CgHSFB1 in the regulation of self-incompatibility in 'Shatian' pummelo.
{Author}: Liu C;Zheng X;Hu J;Xu Q;Wen H;Zhang Z;Liu R;Chen X;Xie Z;Ye J;Deng X;Chai L;
{Journal}: Plant Mol Biol
{Volume}: 114
{Issue}: 4
{Year}: 2024 Jun 23
{Factor}: 4.335
{DOI}: 10.1007/s11103-024-01475-4
{Abstract}: As self-incompatibility is a major issue in pummelo breeding and production, its mechanism in citrus was analyzed to improve breeding efficiency and reduce production costs. Rutaceae belongs to S-RNase type of gametophytic self-incompatibility. While the function of S-RNase/SLF and the mechanism of self-incompatibility have been studied extensively, the transcriptional regulation of S-RNase has been less studied. We performed transcriptome sequencing with the styles of 'Shatian' pummelo on the day of anthesis and 1-5 days before anthesis, and found that the transcript level of S-RNase gradually decreased with flower development. By analyzing differentially expressed genes and correlation with the expression trend of S-RNase, we identified a candidate gene, CgHSFB1, and utilized biochemical experiments such as yeast one-hybrid assay, electrophoretic mobility shift assay and dual-luciferase assay, as well as transient transformation of citrus calli and Citrus microcarpa and demonstrated that CgHSFB1 could directly bind to the S1-RNase promoter and repress the expression of S1-RNase, which is involved in the pummelo self-incompatibility response. In contrast, CgHSFB1 did not bind to the promoter of S2-RNase, and there was specificity in the regulation of S-RNase.