{Reference Type}: Journal Article
{Title}: Protocol for profiling virus-to-host RNA-RNA interactions in infected cells by RIC-seq.
{Author}: Cai Z;Zhao H;Xue Y;
{Journal}: STAR Protoc
{Volume}: 5
{Issue}: 3
{Year}: 2024 Jun 21
暂无{DOI}: 10.1016/j.xpro.2024.103149
{Abstract}: Virus-to-host RNA-RNA interactions directly regulate host mRNA stability and viral replication. However, globally profiling virus-to-host in situ RNA-RNA interactions remains challenging. Here, we present an RNA in situ conformation sequencing (RIC-seq)-based protocol for mapping high-confidence virus-to-host in situ RNA-RNA interactions in infected cells. We detail steps for formaldehyde crosslinking, pCp-biotin labeling, in situ proximity ligation, chimeric RNA enrichment, strand-specific library construction, and data analysis. This protocol allows unbiased identification of virus-to-host RNA-RNA interactions for various RNA viruses and is potentially applicable to DNA virus-derived transcripts. For complete details on the use and execution of this protocol, please refer to Zhao et al.1.