{Reference Type}: Journal Article {Title}: A dataset of trophic state index for nation-scale lakes in China from 40-year Landsat observations. {Author}: Hu M;Ma R;Xue K;Cao Z;Chen X;Xiong J;Xu J;Huang Z;Yu Z; {Journal}: Sci Data {Volume}: 11 {Issue}: 1 {Year}: 2024 Jun 21 {Factor}: 8.501 {DOI}: 10.1038/s41597-024-03506-7 {Abstract}: Trophic state index (TSI) serves as a key indicator for quantifying and understanding the lake eutrophication, which has not been fully explored for long-term water quality monitoring, especially for small and medium inland waters. Landsat satellites offer an effective complement to facilitate the temporal and spatial monitoring of multi-scale lakes. Landsat surface reflectance products were utilized to retrieve the annual average TSI for 2693 lakes over 1 km2 in China from 1984 to 2023. Our method first distinguishes lake types by pixels with a decision tree and then derives relationships between trophic state and algal biomass index. Validation with public reports and existing datasets confirmed the good consistency and reliability. The dataset provides reliable annual TSI results and credible trends for lakes under different area scales, which can serve as a reference for further research and provide convenience for lake sustainable management.