{Reference Type}: Journal Article
{Title}: Multiomics integration-based immunological characterizations of adamantinomatous craniopharyngioma in relation to keratinization.
{Author}: Xu C;Wu J;Ye J;Si Y;Zhang J;Wu B;Pan L;Fu J;Ren Q;Xie S;Tang B;Xiao Y;Hong T;
{Journal}: Cell Death Dis
{Volume}: 15
{Issue}: 6
{Year}: 2024 Jun 21
暂无{DOI}: 10.1038/s41419-024-06840-1
{Abstract}: Although adamantinomatous craniopharyngioma (ACP) is a tumour with low histological malignancy, there are very few therapeutic options other than surgery. ACP has high histological complexity, and the unique features of the immunological microenvironment within ACP remain elusive. Further elucidation of the tumour microenvironment is particularly important to expand our knowledge of potential therapeutic targets. Here, we performed integrative analysis of 58,081 nuclei through single-nucleus RNA sequencing and spatial transcriptomics on ACP specimens to characterize the features and intercellular network within the microenvironment. The ACP environment is highly immunosuppressive with low levels of T-cell infiltration/cytotoxicity. Moreover, tumour-associated macrophages (TAMs), which originate from distinct sources, highly infiltrate the microenvironment. Using spatial transcriptomic data, we observed one kind of non-microglial derived TAM that highly expressed GPNMB close to the terminally differentiated epithelial cell characterized by RHCG, and this colocalization was verified by asmFISH. We also found the positive correlation of infiltration between these two cell types in datasets with larger cohort. According to intercellular communication analysis, we report a regulatory network that could facilitate the keratinization of RHCG+ epithelial cells, eventually causing tumour progression. Our findings provide a comprehensive analysis of the ACP immune microenvironment and reveal a potential therapeutic strategy base on interfering with these two types of cells.