{Reference Type}: Journal Article
{Title}: Evaluating Cationic Nanoparticles as Carriers of Antiviral Nucleic Acids.
{Author}: Jadaa N;Aldor N;Jenik K;Oberhoffner S;Butkowsky C;Tran A;Lewis A;DeWitte-Orr SJ;Poynter S;
{Journal}: Methods Mol Biol
{Volume}: 2813
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3890-3_20
{Abstract}: Nanoparticle carriers enable the multivalent delivery of nucleic acids to cells and protect them from degradation. In this chapter, we present a comprehensive overview of four methodologies: electrophoretic mobility shift assay (EMSA), alamarBlue/CFDA-AM cell viability dyes, fluorescence microscopy, and antiviral assays, which collectively are tools to explore interactions between nucleic acids and nanoparticles, and their biological efficacy. These assays provide insights into binding potential, cytotoxicity, and antiviral efficacy of nucleic acid-based nanoparticle treatments furthering the development of effective antiviral therapeutics.