{Reference Type}: English Abstract
{Title}: [Application of micro-computed tomography (μCT)in quantifying xylem vessels of broadleaved trees].
{Author}: Bai CM;Liang GQ;Fu YL;Han EK;Guo XL;Wang F;
{Journal}: Ying Yong Sheng Tai Xue Bao
{Volume}: 35
{Issue}: 5
{Year}: 2024 May
暂无{DOI}: 10.13287/j.1001-9332.202405.008
{Abstract}: Quantitative analysis of vessel characteristics at the cellular scale is of great significance for understan-ding plant adaptation strategies to environment. The direct grinding combined with stereo-microscope imaging is one of the main approaches to examine the anatomical structure of xylem (conifer tracheid and hardwood vessel) wood structure, which inevitably damages xylem cells, hindering the accurate understanding of anatomical structures. In this study, we applied X-ray micro-computed tomography (μCT) and stereo-microscope technology to quantitatively measure the diameter and area of vessels of seven Canadian broadleaved tree species (Acer saccharum, Betula papyrifera, Fraxinus americana, Ostrya virginiana, Populus grandidentata, Quercus rubra, and Carya cordiformis). We fitted the results by linear model and tested the feasibility of μCT technology in quantifying the vessel size of broadleaved species. We found that the results of the two methods for measuring vessel size were highly similar (R2=0.98). The goodness of fit of the vessel diameter results measured by the two methods for the ring-porous wood species (C. cordiformis, R2=0.98; F. americana, R2=0.96; Q. rubra, R2=0.99) was higher than that of the diffuse-porous wood species (B. papyrifera, R2=0.88; O. virginiana, R2=0.73; A. saccharum, R2=0.68; P. grandiden-tata, R2=0.88). The goodness of fit of small vessels (diameter≤200 μm, R2=0.94) measured by the two methods was higher than that of large vessels (diameter>200 μm, R2=0.92). Thus, the μCT technique provided a new non-destructive detection method for quantifying xylem vessels of broadleaved tree species.<BR>在细胞尺度上定量分析导管特征,对于揭示植物对环境的适应策略具有重要意义。直接打磨样品并结合体视显微镜成像技术是目前研究木质部解剖结构(针叶树管胞和阔叶树导管)的主要方法之一,但仍不可避免损伤木质部细胞,限制了对木质部细胞真实解剖结构的认识。本研究选取了加拿大魁北克南部的糖枫树、北美白桦、美洲白蜡树、美洲铁木、大齿杨、苦味山核桃、北美红橡7个阔叶树种,应用计算机显微断层扫描技术(μCT)和体视显微镜法分别测量各树种的导管直径和面积,采用线性模型对两种方法的测量结果进行数据拟合,探讨采用μCT量化阔叶树导管大小的可行性。结果表明: 对所测定的7个树种,应用两种方法测量导管大小的结果高度相似(R2=0.98)。环孔材树种应用两种方法测量的导管直径结果拟合优度(苦味山核桃R2=0.98,美洲白蜡树R2=0.96,北美红橡R2=0.99)高于散孔材树种(北美白桦R2=0.88,美洲铁木R2=0.73,糖枫树R2=0.68,大齿杨R2=0.88)。应用2种方法测量小导管(直径≤200 μm,R2=0.94)的结果拟合优度高于大导管(直径＞200 μm,R2=0.92)。μCT技术为量化阔叶树种木质部导管提供了一种无损检测新途径。.